Important Links
Call for Papers

call for paper IJCRAS
Submit Article IJCRAS

Why us?
  • Open Access
  • Peer Reviewed
  • Rapid Publication
  • Life time hosting
  • Free promotion service
  • Free indexing service
  • More citations
  • Search engine friendly
Subject Area
  • Life Sciences / Biology
  • Architecture / Building
  • Management
  • Asian Studies
  • Business & Management
  • Chemistry
  • Computer Science
  • Economics & Finance
  • Engineering / Acoustics
  • Environmental Science
  • Agricultural Sciences
  • Pharmaceutical Sciences
  • General Sciences
  • Materials Science
  • Mathematics
  • Medicine
  • Nanotechnology &
  • Nanoscience
  • Nonlinear Science
  • Chaos & Dynamical
  • Systems
  • Physics
  • Social Sciences &
  • Humanities
Title: INFLUENCE OF NACL STRESS AND GAMMA RADIATION ON IN VITRO SEEDS GERMINATION AND SOME PHYSIOLOGICAL PARAMETERS IN CALLUS PRODUCED FROM COWPEA (VIGNA UNGICULATA.L)

Authors: Suad Abduljabbar Alsaedi*, Ekhlas Abdul -Kareem El Kaaby**, Shaimaa A. Yousir, Ashwaq \abed \alrazz\q Aloubaidy

Abstract: Gamma radiation and In Vitro technique were applied in this study to evaluate some morphological and physiological parameters in callus of irradiated Cowpea (Vigna ungiculata. L) seedlings. Local variety seeds of Cowpea were exposed to (0, 25, 50 and 75) Gy of Gamma radiation (60Co). Seeds were sterilized and germinated on Murashige and Skoog (MS) media. Results clarified that, in general, maximum seeds germination 93.0% at 25 Gy whereas maximum seedling height reached (4.53 and 3.40 cm) in addition to number of leaves (3.57,2.90) were observed at 25 and 50 Gy respectively.  All mutagenic doses caused a significant reduction in photosynthetic chlorophyll pigment compared with control (non irradiated seeds). Callus was initiated from shoot tip explants and exposed to (NaCl) stress at the levels (6, 8 and 10 ds.l-1). 4 weeks later, Proline, CHO and minerals were estimated. In general, increasing gamma radiation doses at 50 up to 75 Gy and NaCl at the level 10 ds. m-1 increased accumulation of Na+ and Clwhereas highest accumulation of K+ was found with 75 Gy when combined with 6 and 8 ds.  m-1. Moreover, highest content of CHO (8.75 mg. g-1) was observed in 25Gy combined with the level 10 ds.m-1 and Proline (26.46 mg. g-1) at 50 Gy combined with the level 10 ds.m-1.

Keywords: Chlorophyll, Proline, CHO, 60Co, Na+, K+

PDF Download

 

Announcements
Author Information
Benefits
  • Rapid Publishing
  • Professional publishing practices
  • Indexing in leading database
  • High level of citation
  • High-Quality reader base
  • High-level author support
Plagiarism

IJCRAS is following an instant policy on rejecting received papers with a plagiarism rate of more than 20%. So, All authors and contributors must check their papers before submission to make assurance of following our anti-plagiarism policies.

Modes of Payment

PayPal IJCRAS

Bank Account Transfer

Western Union IJCRAS