Title: ANTIMICROBIAL AND CHEMICAL COMPOSITION OF ESSENTIAL OIL OF MONODORA MYRISTICA SEEDS ON SOME CLINICAL BACTERIA ISOLATES
Authors: Musbau, S., Asiru, R.A. and Muhammad, Y.
Abstract: Monodora myristica seeds were obtained from Damaturu main market in Yobe State, ground into fine powder and stored in an air tight plastic container at room temperature until when used. Essential oils were extracted using Soxhlet extraction method and determinations of active component were carried out using gas chromatography-mass spectroscopy (GC-MS) technique. Microbial isolates were obtained from clinical samples obtained from hospital in Yobe state. Identification and characterization of isolates were carried out following standard microbiological procedures. The antimicrobial activity was determined using agar well diffusion method. Data obtained were subjected to a one-way analysis of variance. Significant means were separated using Duncan Multiple Range Test at 95% confidence level. The result showed that M. myristica oil contained eleven components which were mainly Indole-3-ethanol, acetate with the highest peak (6.22). Results of the antibacterial activity of the oil extracts (hexane, chloroform and ethanolic) reveal that all the extracts at different concentrations were active against the test isolates namely Proteus vulgaris, Pseudomonas aeruginosa, Escherichia coli, Shigella boydii and Klebsiella oxytoca. The minimum inhibitory concentration (MIC) values for all the three oil extracts ranged between 0.5 and 1.0%, while the minimum bacterial concentration (MBC) values for all the three oil extraction ranged between 1.0 and above 1.5%. The expressed antimicrobial activity is a demonstration of the efficacy of the oil against clinical isolates.
Keywords: Essential oils, antimicrobial activity, Monodora myristica, and agar well diffusion method.
PDF Download