The weed Hyptis suaveolens L. Poit.in East Nusa Tenggara can be easily found, it can grow anywhere, such as on the side of the road or even on dry land. In Kupang City, is known as a weed and nuisance plant that grows wild on roadsides or former fields. The main aim of this research is to determine the content of secondary metabolite compounds in the roots, stems, and leaves of H. suaveolens. The method used was phytochemical screening by testing the content of secondary metabolite compounds in the roots, stems, and leaves of H. suaveolens. The research found that the roots, stems, and leaves of H. suaveolens positively contain secondary metabolite compounds, namely alkaloids, flavonoids, tannins, terpenoids, steroids, saponins, and quinones. Therefore, this local species was potentially to be used as basic ingredients for a medicinal plant as well as as a natural insecticide. However, further research was needed to test the antibacterial activity, and its effectiveness as a medicinal plant, and to confirm the efficacy and determine safe doses as natural medicines.