This study was to analyze the distribution patterns of phytoplankton and zooplankton at different times and depths in the fishing area of Kupang Bay. The method used in this study was the trap method for plankton sampling using plankton nets and the Swdwich Rafer Counting Cell (SRCC) method to identify and enumerate plankton types. The results of the study showed that the density of phytoplankton and zooplankton at different times and depths in different fishing locations was different, namely the density of phytoplankton was higher than zooplankton. On a full moon, the density of phytoplankton was higher than zooplankton and in the the middle part, the density of phytoplankton and zooplankton were higher than the surface and bottom. The distribution pattern of phytoplankton and zooplankton at different times and depths was a uniform and regular distribution. Environmental conditions at the fishing area of Kupang Bay were classified as good or suitable for the survival or growth of plankton. It is necessary to continue preserve the coastal and marine environment of Kupang Bay to maintain the potential of the area as a fishing area for local fishermen